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KMID : 0385519980110050404
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Analytical Science & Technology
1998 Volume.11 No. 5 p.404 ~ p.408
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Separation of 20(R&S) Prosapogenin Isomers of Ginsenoside - Rg₂ and - Rg₃ from Ginseng Saponins by Reversed - Phase High Performance Liquid Chromatography
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정승일/Jeong SI
김천석/이용구/이호섭/김일광/Kim CS/Lee YG/Lee HS/Kim IK
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Abstract
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Using a reversed-phase high performance liquid chromatography, the separation of 20(S)-, 20(R)-prosapogenin stereo-isomers of ginsenoside-Rg₂ and of ginsenoside-Rg₃ in ginseng saponins has been carried out with binary solvent system. The optimum conditions for the isomer separation are as following: Nova-Pak®C_(18) (Waters, 3.9×150 ㎜) column, CH₃CN/CH₃CN (100:8, v/v) binary solvent system and the flow rate was 1.7 mL/min. The stereoisomers were separated with change of the mixture ratio of the solvent system, the solvent elution by gradient program, and then detected at 203 ㎚ of UV detector. The simultaneous separation of mixture that were the Rg₂, Rg₃ isomers was easily performed in nonpolar solvent for Rg₂, polar solvent for Rg₃ at the same optimum conditions.
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